West Ham United will travel to Southampton for their Emirates FA Cup fifth round tie on Wednesday 2 March.
The game, which will be broadcast live on the BBC Red Button and iPlayer, will be played at St Mary's Stadium, with kick off at 7.30pm.
The Hammers secured their place in the last 16 in thrilling fashion last weekend, scoring in added-time at the end of normal time and extra-time to edge out National League North side Kidderminster Harriers at Aggborough Stadium.
The three-times winners had previously defeated fellow Premier League side Leeds United 2-0 in the third round at London Stadium in January.
Southampton also needed extra-time to see off EFL Championship opponents Coventry City 2-1 at St Mary's on Saturday, having won 3-2 at Swansea City in the third round.
We have won all three previous meetings with Southampton in the FA Cup.
The last time we faced the Saints in the competition was in the third round back in 1975, when goals from Frank Lampard and Bobby Gould secured a 2-1 win. West Ham went on to lift the trophy for the second time in our history a few months later.
Prior to that, we also won a fourth round second replay (at Villa Park!) on the way to our first appearance in the final in 1923, having also got past Southampton 3-1 in the first round in 1920 in our first season as a League club.
Ticket details for this season's fifth-round tie will be announced in due course, with the game set to be decided on the night with no replays at this stage of the competition.